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1

Breaking the mirror: l -Amino acid deaminase, a novel stereoselective biocatalyst

Molla, Gianluca, Melis, Roberta, Pollegioni, Loredano

Journal:

Biotechnology Advances

Year:

2017

Language:

english

File:

PDF, 1.44 MB

english, 2017

2

In vitro evolution of an l -amino acid deaminase active on l -1-naphthylalanine

Melis, Roberta, Rosini, Elena, Pirillo, Valentina, Pollegioni, Loredano, Molla, Gianluca

Journal:

Catalysis Science & Technology

Year:

2018

Language:

english

File:

PDF, 2.61 MB

english, 2018

3

Enzymatic Conversion of Unnatural Amino Acids by Yeast D-Amino Acid Oxidase

Antonio Caligiuri, Paola D'Arrigo, Elena Rosini, Davide Tessaro, Gianluca Molla, Stefano Servi, Loredano Pollegioni

Journal:

Advanced Synthesis & Catalysis

Year:

2006

Language:

english

File:

PDF, 334 KB

english, 2006

4

ChemInform Abstract: Chemoenzymatic Deracemization Methods

Davide Tessaro, Gianluca Molla, Loredano Pollegioni, Stefano Servi

Journal:

Year:

2010

File:

PDF, 11 KB

2010

5

On the mechanism of Rhodotorula gracilis d-amino acid oxidase: role of the active site serine 335

Angelo Boselli, Luciano Piubelli, Gianluca Molla, Silvia Sacchi, Mirella S. Pilone, Sandro Ghisla, Loredano Pollegioni

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2004

Language:

english

File:

PDF, 411 KB

english, 2004

6

The role of tyrosines 223 and 238 in Rhodotorula gracilis d-amino acid oxidase catalysis: Interpretation of double mutations

Silvia Sacchi, Angelo Boselli, Viviana Job, Mirella S. Pilone, Loredano Pollegioni, Gianluca Molla

Journal:

Enzyme and Microbial Technology

Year:

2006

Language:

english

File:

PDF, 205 KB

english, 2006

7

CASCAT: The Power of The Combined Protein Engineering Approach: Evolution of A Glycine Oxidase for A Novel Mechanism of Glyphosate Tolerance

Pollegioni Loredano, Pedotti Mattia, Rosini Elena, Molla Gianluca, Moschetti Tommaso

Journal:

Journal of Biotechnology

Year:

2010

Language:

english

File:

PDF, 91 KB

english, 2010

8

Evolution of Chymotrypsin-Like Enzymes for Specific Hydrolytic Bioconversions of Industrial Interest

Volonté Federica, Tessaro Davide, Molla Gianluca, D’Arrigo Paola, Servi Stefano, Pollegioni Loredano

Journal:

Journal of Biotechnology

Year:

2010

Language:

english

File:

PDF, 64 KB

english, 2010

9

Production of Evolved Variants of D-Amino Acid Oxidase for A Cancer Enzyme Therapy

Elena, Rosini, Gianluca, Molla, Silvia, Sacchi, Loredano, Pollegioni

Journal:

Journal of Biotechnology

Year:

2010

Language:

english

File:

PDF, 73 KB

english, 2010

10

New biotech applications from evolved D-amino acid oxidases

Loredano Pollegioni, Gianluca Molla

Journal:

Trends in Biotechnology

Year:

2011

Language:

english

File:

PDF, 1.05 MB

english, 2011

11

Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli

Viviana Job, Gianluca Molla, Mirella S. Pilone, Loredano Pollegioni

Journal:

Year:

2002

Language:

english

File:

PDF, 333 KB

english, 2002

12

Kinetic mechanisms of glycine oxidase from Bacillus subtilis

Gianluca Molla, Laura Motteran, Viviana Job, Mirella S. Pilone, Loredano Pollegioni

Journal:

Year:

2003

Language:

english

File:

PDF, 405 KB

english, 2003

13

Redox potentials and their pH dependence of D-amino-acid oxidase of Rhodotorula gracilis and Trigonopsis variabilis

Loredano Pollegioni, Davide Porrini, Gianluca Molla, Mirella S. Pilone

Journal:

Year:

2000

Language:

english

File:

PDF, 771 KB

english, 2000

14

Production of recombinant cholesterol oxidase containing covalently bound FAD inEscherichia coli

Federica Volontè, Loredano Pollegioni, Gianluca Molla, Luca Frattini, Flavia Marinelli, Luciano Piubelli

Journal:

BMC Biotechnology

Year:

2010

Language:

english

File:

PDF, 1.10 MB

english, 2010

15

Effect of ligand binding on human D-amino acid oxidase: Implications for the development of new drugs for schizophrenia treatment

Laura Caldinelli, Gianluca Molla, Luisa Bracci, Barbara Lelli, Silvia Pileri, Pamela Cappelletti, Silvia Sacchi, Loredano Pollegioni

Journal:

Protein Science

Year:

2010

Language:

english

File:

PDF, 408 KB

english, 2010

16

Relevance of weak flavin binding in human D-amino acid oxidase

Laura Caldinelli, Gianluca Molla, Silvia Sacchi, Mirella S. Pilone, Loredano Pollegioni

Journal:

Protein Science

Year:

2009

Language:

english

File:

PDF, 229 KB

english, 2009

17

Regulation of D-amino acid oxidase expression in the yeast Rhodotorula gracilis

Gianluca Molla, Laura Motteran, Luciano Piubelli, Mirella S. Pilone, Loredano Pollegioni

Journal:

Year:

2003

Language:

english

File:

PDF, 169 KB

english, 2003

18

Properties and applications of microbialD-amino acid oxidases: current state and perspectives

Loredano Pollegioni, Gianluca Molla, Silvia Sacchi, Elena Rosini, Roberto Verga, Mirella S. Pilone

Journal:

Applied Microbiology and Biotechnology

Year:

2008

Language:

english

File:

PDF, 596 KB

english, 2008

19

Tryptophan 243 affects interprotein contacts, cofactor binding and stability in D-amino acid oxidase from Rhodotorula gracilis

Laura Caldinelli, Gianluca Molla, Mirella S. Pilone, Loredano Pollegioni

Journal:

Year:

2006

Language:

english

File:

PDF, 292 KB

english, 2006

20

Optimization of d-amino acid oxidase for low substrate concentrations – towards a cancer enzyme therapy

Elena Rosini, Loredano Pollegioni, Sandro Ghisla, Roberto Orru, Gianluca Molla

Journal:

Year:

2009

Language:

english

File:

PDF, 509 KB

english, 2009

21

Cholesterol oxidase: biotechnological applications

Loredano Pollegioni, Luciano Piubelli, Gianluca Molla

Journal:

Year:

2009

Language:

english

File:

PDF, 682 KB

english, 2009

22

On the reaction of d-amino acid oxidase with dioxygen: O2 diffusion pathways and enhancement of reactivity

Elena Rosini, Gianluca Molla, Sandro Ghisla, Loredano Pollegioni

Journal:

Year:

2011

Language:

english

File:

PDF, 468 KB

english, 2011

23

Is rat an appropriate animal model to study the involvement of d-serine catabolism in schizophrenia? insights from characterization of d-amino acid oxidase

Luca F. Frattini, Luciano Piubelli, Silvia Sacchi, Gianluca Molla, Loredano Pollegioni

Journal:

Year:

2011

Language:

english

File:

PDF, 360 KB

english, 2011

24

Catalytic Properties of d-Amino Acid Oxidase in Cephalosporin C Bioconversion: A Comparison between Proteins from Different Sources

Loredano Pollegioni, Laura Caldinelli, Gianluca Molla, Silvia Sacchi, Mirella S. Pilone

Journal:

Biotechnology Progress

Year:

2004

Language:

english

File:

PDF, 108 KB

english, 2004

25

Evolution of an acylase active on cephalosporin C

Loredano Pollegioni, Simona Lorenzi, Elena Rosini, Giorgia Letizia Marcone, Gianluca Molla, Roberto Verga, Walter Cabri, Mirella S. Pilone

Journal:

Protein Science

Year:

2005

Language:

english

File:

PDF, 963 KB

english, 2005

26

Relevance of the flavin binding to the stability and folding of engineered cholesterol oxidase containing noncovalently bound FAD

Laura Caldinelli, Stefania Iametti, Alberto Barbiroli, Dimitrios Fessas, Francesco Bonomi, Luciano Piubelli, Gianluca Molla, Loredano Pollegioni

Journal:

Protein Science

Year:

2008

Language:

english

File:

PDF, 525 KB

english, 2008

27

Overexpression of a bacterial chymotrypsin: Application for l-amino acid ester hydrolysis

Federica Volontè, Ines Pisanelli, Paola D’Arrigo, Fiorenza Viani, Gianluca Molla, Stefano Servi, Loredano Pollegioni

Journal:

Enzyme and Microbial Technology

Year:

2011

Language:

english

File:

PDF, 965 KB

english, 2011

28

Activity of yeast d-amino acid oxidase on aromatic unnatural amino acids

Antonio Caligiuri, Paola D’Arrigo, Elena Rosini, Giuseppe Pedrocchi-Fantoni, Davide Tessaro, Gianluca Molla, Stefano Servi, Loredano Pollegioni

Journal:

Journal of Molecular Catalysis B: Enzymatic

Year:

2008

Language:

english

File:

PDF, 307 KB

english, 2008

29

Overexpression inEscherichia coliof a Recombinant ChimericRhodotorula gracilisd-Amino Acid Oxidase

Gianluca Molla, Cristina Vegezzi, Mirella S. Pilone, Loredano Pollegioni

Journal:

Protein Expression and Purification

Year:

1998

Language:

english

File:

PDF, 121 KB

english, 1998

30

Investigating the role of active site residues of Rhodotorula gracilis d-amino acid oxidase on its substrate specificity

Angelo Boselli, Luciano Piubelli, Gianluca Molla, Mirella S. Pilone, Loredano Pollegioni, Silvia Sacchi

Journal:

Year:

2007

Language:

english

File:

PDF, 476 KB

english, 2007

31

Glycine oxidase from Bacillus subtilis: Role of Histidine 244 and Methionine 261

Angelo Boselli, Elena Rosini, Giorgia Letizia Marcone, Silvia Sacchi, Laura Motteran, Mirella S. Pilone, Loredano Pollegioni, Gianluca Molla

Journal:

Year:

2007

Language:

english

File:

PDF, 521 KB

english, 2007

32

Catalytic and redox properties of glycine oxidase from Bacillus subtilis

Mattia Pedotti, Sandro Ghisla, Laura Motteran, Gianluca Molla, Loredano Pollegioni

Journal:

Year:

2009

Language:

english

File:

PDF, 690 KB

english, 2009

33

FAD binding in glycine oxidase from Bacillus subtilis

Laura Caldinelli, Mattia Pedotti, Laura Motteran, Gianluca Molla, Loredano Pollegioni

Journal:

Year:

2009

Language:

english

File:

PDF, 929 KB

english, 2009

34

Characterization of human d-amino acid oxidase

Gianluca Molla, Silvia Sacchi, Mariagrazia Bernasconi, Mirella S. Pilone, Kiyoshi Fukui, Loredano Pollegioni

Journal:

Year:

2006

Language:

english

File:

PDF, 157 KB

english, 2006

35

Expression in Escherichia coli and in vitro refolding of the human protein pLG72

Gianluca Molla, Mariagrazia Bernasconi, Silvia Sacchi, Mirella S. Pilone, Loredano Pollegioni

Journal:

Protein Expression and Purification

Year:

2006

Language:

english

File:

PDF, 279 KB

english, 2006

36

Optimization of glutaryl-7-aminocephalosporanic acid acylase expression in E. coli

Federica Volontè, Flavia Marinelli, Luciano Gastaldo, Silvia Sacchi, Mirella S. Pilone, Loredano Pollegioni, Gianluca Molla

Journal:

Protein Expression and Purification

Year:

2008

Language:

english

File:

PDF, 449 KB

english, 2008

37

Optimization of human d-amino acid oxidase expression in Escherichia coli

Diego Romano, Gianluca Molla, Loredano Pollegioni, Flavia Marinelli

Journal:

Protein Expression and Purification

Year:

2009

Language:

english

File:

PDF, 408 KB

english, 2009

38

Expression in Escherichia coli of the catalytic domain of human proline oxidase

Elena Tallarita, Loredano Pollegioni, Stefano Servi, Gianluca Molla

Journal:

Protein Expression and Purification

Year:

2012

Language:

english

File:

PDF, 1.04 MB

english, 2012

39

Identification and role of ionizing functional groups at the active center of Rhodotorula gracilis D-amino acid oxidase

Loredano Pollegioni, Christopher M. Harris, Gianluca Molla, Mirella S. Pilone, Sandro Ghisla

Journal:

Year:

2001

Language:

english

File:

PDF, 158 KB

english, 2001

40

Conversion of the dimeric D-amino acid oxidase from Rhodotorula gracilis to a monomeric form. A rational mutagenesis approach

Luciano Piubelli, Laura Caldinelli, Gianluca Molla, Mirella S Pilone, Loredano Pollegioni

Journal:

Year:

2002

Language:

english

File:

PDF, 264 KB

english, 2002

41

Yeast d-Amino Acid Oxidase: Structural Basis of its Catalytic Properties

Loredano Pollegioni, Kay Diederichs, Gianluca Molla, Stephan Umhau, Wolfram Welte, Sandro Ghisla, Mirella S. Pilone

Journal:

Journal of Molecular Biology

Year:

2002

Language:

english

File:

PDF, 1.67 MB

english, 2002

42

A biosensor for all d-amino acids using evolved d-amino acid oxidase

Elena Rosini, Gianluca Molla, Carlo Rossetti, Mirella S. Pilone, Loredano Pollegioni, Silvia Sacchi

Journal:

Journal of Biotechnology

Year:

2008

Language:

english

File:

PDF, 628 KB

english, 2008

43

Cloning, sequencing and expression in E. coli of a d-amino acid oxidase cDNA from Rhodotorula gracilis active on cephalosporin C

Loredano Pollegioni, Gianluca Molla, Stefano Campaner, Enzo Martegani, Mirella S Pilone

Journal:

Journal of Biotechnology

Year:

1997

Language:

english

File:

PDF, 289 KB

english, 1997

44

Characterization of the Covalently Bound Anionic Flavin Radical in Monoamine Oxidase A by Electron Paramagnetic Resonance

Kay, Christopher W. M., El Mkami, Hassane, Molla, Gianluca, Pollegioni, Loredano, Ramsay, Rona R.

Journal:

Journal of the American Chemical Society

Year:

2007

Language:

english

File:

PDF, 160 KB

english, 2007

45

Cephalosporin C acylase: dream and(/or) reality

Pollegioni, Loredano, Rosini, Elena, Molla, Gianluca

Journal:

Applied Microbiology and Biotechnology

Year:

2013

Language:

english

File:

PDF, 905 KB

english, 2013

46

A thermostable L-aspartate oxidase: a new tool for biotechnological applications

Bifulco, Davide, Pollegioni, Loredano, Tessaro, Davide, Servi, Stefano, Molla, Gianluca

Journal:

Applied Microbiology and Biotechnology

Year:

2013

Language:

english

File:

PDF, 426 KB

english, 2013

47

l-Amino acid oxidase as biocatalyst: a dream too far?

Pollegioni, Loredano, Motta, Paolo, Molla, Gianluca

Journal:

Applied Microbiology and Biotechnology

Year:

2013

Language:

english

File:

PDF, 665 KB

english, 2013

48

Characterization of human DAAO variants potentially related to an increased risk of schizophrenia

Caldinelli, Laura, Sacchi, Silvia, Molla, Gianluca, Nardini, Marco, Pollegioni, Loredano

Journal:

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease

Year:

2013

Language:

english

File:

PDF, 1.62 MB

english, 2013

49

Multistep enzyme catalysed deracemisation of 2-naphthyl alanine

Caligiuri, Antonio, D'Arrigo, Paola, Gefflaut, Thierry, Molla, Gianluca, Pollegioni, Loredano, Rosini, Elena, Rossi, Cristina, Servi, Stefano

Journal:

Biocatalysis and Biotransformation

Year:

2006

Language:

english

File:

PDF, 94 KB

english, 2006

50

Novel biosensors based on optimized glycine oxidase

Rosini, Elena, Piubelli, Luciano, Molla, Gianluca, Frattini, Luca, Valentino, Mattia, Varriale, Antonio, D'Auria, Sabato, Pollegioni, Loredano

Journal:

Year:

2014

Language:

english

File:

PDF, 956 KB

english, 2014

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